Magnetic hydrogel scaffold based on hyaluronic acid/chitosan and gelatin natural polymers

• Published in: Scientific reports Vol. 14; no. 1; pp. 28206 - 15
• Main Authors: Abou-Okeil, Ashraf, Refaei, Rakia, Moustafa, Shaimaa E., Ibrahim, Hassan M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15-11-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/154; 639/301; 639/925; Antibacterial activity; Bacteria; Biomaterials; Chitosan; Ciprofloxacin; Controlled release; E coli; Extracellular matrix; Gelatin; Glutaraldehyde; Gram-negative bacteria; Gram-positive bacteria; Humanities and Social Sciences; Hyaluronic; Hyaluronic acid; Hydrogels; Infrared spectroscopy; Iron oxide; Iron oxides; Magnetic hydrogel; Mechanical properties; multidisciplinary; Nanoparticles; Polymers; Porosity; Scaffold; Scanning electron microscopy; Science; Science (multidisciplinary); Tissue engineering; Iron oxide; Scaffold; Chitosan; Magnetic hydrogel; Gelatin; Hyaluronic
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-78696-6

Owing to their native extracellular matrix-like features, magnetic hydrogels have been proven to be promising biomaterials as tissue engineering templates In the present work, magnetic hydrogels scaffold based on chitosan, gelatin, hyaluronic acid, containing Fe
3
O
4
as magnetic nanoparticles (IONPs) were prepared. The prepared hydrogels were loaded with ciprofloxacin hydrochloride as a model drug. The magnetic hydrogel was prepared using different volumes of chitosan, 1%, gelatin, 10%, and hyaluronic acid, 1% in glutaraldehyde as the crosslinking agent and Fe
3
O
4
as magnetic nanoparticles. The hydrogel scaffold and magnetic scaffold hydrogel samples were characterized by scanning electron microscopy (SEM), vibrating sample magnetometry (VSM), and Fourier-transform infrared spectroscopy (FTIR). The porosity, mechanical properties, swelling degree, and antibacterial activity of the hydrogel scaffold were also determined as well as the drug release profiles of the hydrogels. SEM imaging revealed that the magnetic hydrogel scaffold showed a relatively rough morphology with an irregular surface. The data obtained indicated that the hydrogel surface has three-dimensional porous microstructures and the porosity varied depending on the hydrogel formulation. The breaking load of the hydrogel scaffold increased from 1.361 Kgf to 4.98 Kgf by increasing the glutaraldehyde concentration from 0.2 mL to 0.8 mL. Swelling degree values in water were from 250 to 2000% after 24 h. The antibacterial activity of the hydrogel scaffold ranged from 54% to about 97% for Gram-positive bacteria (
S. aureus
) and from about 26–92% for Gram-negative bacteria (
E. coli
). The ciprofloxacin hydrochloride loaded hydrogel has a sustained release of ciprofloxacin hydrochloride over 10 h. The presence of IONPs gave a faster release of ciprofloxacin hydrochloride.