Conserved biophysical features of the CaV2 presynaptic Ca2+ channel homologue from the early-diverging animal Trichoplax adhaerens

• Published in: The Journal of biological chemistry Vol. 295; no. 52; pp. 18553 - 18578
• Main Authors: Gauberg, Julia, Abdallah, Salsabil, Elkhatib, Wassim, Harracksingh, Alicia N., Piekut, Thomas, Stanley, Elise F., Senatore, Adriano
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 25-12-2020; American Society for Biochemistry and Molecular Biology
• Subjects: calcium channel; CaV2 presynaptic Ca2+ channels; evolution; exocytosis; G protein; Gβγ inhibition; ion channel; Molecular Biophysics; patch clamp; patch clamp electrophysiology; pharmacology; synapse; synapse evolution; Trichoplax adhaerens; Voltage-gated Ca2+ channels; calcium channel; patch clamp; Voltage-gated Ca2+ channels; CaV2 presynaptic Ca2+ channels; synapse; evolution; Trichoplax adhaerens; synapse evolution; Gβγ inhibition; ion channel; Gβγ-inhibition; patch clamp electrophysiology; pharmacology; exocytosis; G protein
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.RA120.015725

The dominant role of CaV2 voltage-gated calcium channels for driving neurotransmitter release is broadly conserved. Given the overlapping functional properties of CaV2 and CaV1 channels, and less so CaV3 channels, it is unclear why there have not been major shifts toward dependence on other CaV channels for synaptic transmission. Here, we provide a structural and functional profile of the CaV2 channel cloned from the early-diverging animal Trichoplax adhaerens, which lacks a nervous system but possesses single gene homologues for CaV1–CaV3 channels. Remarkably, the highly divergent channel possesses similar features as human CaV2.1 and other CaV2 channels, including high voltage–activated currents that are larger in external Ba2+ than in Ca2+; voltage-dependent kinetics of activation, inactivation, and deactivation; and bimodal recovery from inactivation. Altogether, the functional profile of Trichoplax CaV2 suggests that the core features of presynaptic CaV2 channels were established early during animal evolution, after CaV1 and CaV2 channels emerged via proposed gene duplication from an ancestral CaV1/2 type channel. The Trichoplax channel was relatively insensitive to mammalian CaV2 channel blockers ω-agatoxin-IVA and ω-conotoxin-GVIA and to metal cation blockers Cd2+ and Ni2+. Also absent was the capacity for voltage-dependent G-protein inhibition by co-expressed Trichoplax Gβγ subunits, which nevertheless inhibited the human CaV2.1 channel, suggesting that this modulatory capacity evolved via changes in channel sequence/structure, and not G proteins. Last, the Trichoplax channel was immunolocalized in cells that express an endomorphin-like peptide implicated in cell signaling and locomotive behavior and other likely secretory cells, suggesting contributions to regulated exocytosis.