Stimulated Brillouin scattering flow cytometry

Stimulated Brillouin scattering flow cytometry

We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of 5 ms, which marks a 1...

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Bibliographic Details
Published in:Biomedical optics express Vol. 15; no. 10; pp. 6024 - 6035
Main Authors: Rosvold, Jake R, Zanini, Giulia, Handler, Chenchen, Frank, Eric, Li, Jiarui, Vitolo, Michele I, Martin, Stuart S, Scarcelli, Giuliano
Format: Journal Article
Language:English
Published: United States Optica Publishing Group 01-10-2024
Online Access:Get full text
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Summary:We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of 5 ms, which marks a 10x improvement compared to previous demonstrations of spontaneous Brillouin scattering flow cytometry. We experimentally validate our stimulated Brillouin scattering flow cytometer by measuring cell populations of normal breast epithelial cells and metastatic breast epithelial cancer cells.
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ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.537602