Transcriptome Analysis of a Wine Yeast Strain with a Deletion of the CAR1 Arginase Gene

• Published in: Microbiology (New York) Vol. 92; no. Suppl 1; pp. S93 - S96
• Main Authors: Mardanov, A. V., Vasyagin, E. A., Mardanova, E. S., Beletsky, A. V., Shalamitskiy, M. Yu, Tanashchuk, T. N., Urakov, V. N., Kushnirov, V. V., Rakitin, A. L., Ravin, N. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-12-2023; Springer Nature B.V
• Subjects: Arginase; Biomedical and Life Sciences; Carcinogens; Ethyl carbamate; Fermentation; Gene deletion; Genes; Genomes; Kinases; Life Sciences; MAP kinase; Medical Microbiology; Metabolic pathways; Microbiology; Next-generation sequencing; Ornithine-aminotransferase; Short Communications; Transcription; Transcriptomes; Urea; Wine; Yeast; genome editing; ethyl carbamate; transcriptome; wine; arginase
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261723603901

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Ethyl carbamate, a potential carcinogen, is formed in wine as a result of a chemical reaction between ethanol and urea during fermentation and storage. Using genome editing methods, a modified strain of wine yeast
Saccharomyces cerevisiae
was constructed, with a deletion of the
CAR1
arginase gene, leading to a decrease in the formation of urea from arginine. Transcriptome analysis by high-throughput sequencing identified 738 genes whose transcription level reliably changed more than twofold as a result of deletion of the
CAR1
gene. Expression of most genes of the central metabolic pathways in the
CAR1
deletion strain was decreased; increased transcription levels were observed for the Map kinase signaling cascades. Among the genes of arginine metabolism pathways, the level of transcription of the ornithine aminotransferase gene
CAR2
increased significantly. The wine material obtained using a strain with a deletion of the
CAR1
gene under micro-winemaking conditions contained 25% less urea than in the case of using an unmodified strain. The resulting strain of wine yeast, having lower urea levels, may be promising for winemaking.