Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost

• Published in: International journal of systematic and evolutionary microbiology Vol. 52; no. 6; pp. 2251 - 2255
• Main Authors: Sung, M. H, Kim, H, Bae, J. W, Rhee, S. K, Jeon, C. O, Kim, K, Kim, J. J, Hong, S. P, Lee, S. G, Yoon, J. H, Park, Y. H, Baek, D. H
• Format: Journal Article
• Language: English
• Published: Soc General Microbiol 01-11-2002
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.02181-0

M. H. Sung, H. Kim, J. W. Bae, S. K. Rhee, C. O. Jeon, K. Kim, J. J. Kim, S. P. Hong, S. G. Lee, J. H. Yoon, Y. H. Park and D. H. Baek
Microbial Genomics Lab., Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon 305-600, Korea
A thermophilic, spore-forming rod isolated from hay compost in Korea was
subjected to a taxonomic study. The micro-organism, designated strain
SK-1(T), was identified as being aerobic, Gram-positive, motile and
rod-shaped. Growth of the isolate was observed at 45--70 degrees C (optimum
60 degrees C) and pH 6.0--9.0 (optimum pH 7.5). The G+C content of the
genomic DNA was 43.9 mol%. Chemotaxonomic characteristics of the isolate
included the presence of meso-diaminopimelic acid in the cell wall and
iso-C(15:0) and iso-C(17:0) as the major cellular fatty acids. The
predominant isoprenoid quinone was MK-7. The chemotaxonomic characteristics
of strain SK-1(T) were the same as those of the genus Geobacillus.
Phylogenetic analysis based on 16S rDNA sequences showed that strain
SK-1(T) is most closely related to Geobacillus thermoglucosidasius.
However, the phenotypic properties of strain SK-1(T) were clearly different
from those of G. thermoglucosidasius. The level of DNA--DNA relatedness
between strain SK-1(T) and the type strain of G. thermoglucosidasius was
27%. On the basis of the phenotypic traits and molecular systematic data,
strain SK-1(T) represents a novel species within the genus Geobacillus, for
which the name Geobacillus toebii sp. nov. is proposed. The type strain is
strain SK-1(T) (=KCTC 0306BP(T)=DSM 14590(T)).