Lighting Intensity Affects the Fatty Acid Composition of Total Lipids of Basil Leaves and Roots (Ocimum basilicum L.)

• Published in: Russian journal of plant physiology Vol. 70; no. 5
• Main Authors: Ivanova, T. V., Voronkov, A. S.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-10-2023; Springer Nature B.V
• Subjects: Biomass; Biomedical and Life Sciences; Chlorophyll; Composition; Esterification; Fatty acid composition; Fatty acids; Illumination; Leaves; Life Sciences; Lighting; Lipids; Luminous intensity; Moisture content; Ocimum basilicum; Parameter sensitivity; Plant Physiology; Plant roots; Plant Sciences; Plants; Research Papers; Roots; Species composition; Water content; very-long-chain fatty acids; adaptation; light intensity; desaturase activity
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443723600794

The vegetative organs of common basil (
Ocimum basilicum
L.) grown in water culture at different levels of illumination (50, 100, and 150 μmol PAR quanta/(m
2
s)) for 21 days were studied. In the work, the Purple Velvet basil variety, which is popular in agriculture and has intensely colored leaves was used. The biomass, water content, and composition of fatty acids (FA) of total lipids in the leaves and roots of plants and the content of malondialdehyde and chlorophylls (
a, b
) in the aerial parts of plants were determined. The sensitivity of the FA composition and morphophysiological parameters (biomass and chlorophyll content in leaves) of
O. basilicum
plants to the intensity of illumination was shown. The greatest effect was caused by the illumination intensity mode of 150 µmol/(m
2
s). Species composition of
O. basilicum
FA under all lighting conditions was wider in root lipids; however, more pronounced changes in the qualitative composition of FAs were observed in leaves. Photodependent regulation of FA component composition of
O. basilicum
manifested itself in an increase in the proportion of unsaturated FAs, especially polyene ones, which led to an increase in the unsaturation index (UI) of esterified lipid FAs. The highest UI values were obtained with illumination of 150 µmol/(m
2
s). At the same time, with an increase in the illumination intensity, a significant increase in the activity of ω-3 and ω-9-desaturases occurred, which indicates in favor of their light-dependent activation. Thus, increasing the intensity of lighting to certain values directly proportionally affects the physiological parameters of
O. basilicum.
The maximum indicators of productive growth and the implementation of adaptive mechanisms of green and underground parts of basil plants correspond to illumination of 150 µmol/(m
2
s).