Biocatalytic Conversion of Semi-Finished Hardwood into Sugars: Enzymatic Hydrolysis at High Concentrations of the Substrate

• Published in: Catalysis in industry Vol. 16; no. 1; pp. 102 - 109
• Main Authors: Semenova, M. V., Telitsin, V. D., Rozhkova, A. M., Kondratyeva, E. G., Shashkov, I. A., Satrutdinov, A. D., Gareeva, Ya. A., Moseev, V. G., Kryazhev, A. M., Sinitsyn, A. P.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-03-2024; Springer Nature B.V
• Subjects: Biocatalysis; Bleaching; Catalysis; Cellulase; Cellulose; Chemistry; Chemistry and Materials Science; Enzymes; Glucose; Glucosidase; Hydrolysis; Paper products; Paperboard; Proteins; Substrates; Sugar; Xylose; cellulases; semi-bleached sulfate hardwood pulp; enzymatic hydrolysis; high substrate concentration
• ISSN: 2070-0504; 2070-0555
• DOI: 10.1134/S2070050424010070

Exhaustive enzymatic hydrolysis is performed for semi-bleached sulfate hardwood cellulose (a semi-finished pulp and paper product) at ultra-high concentrations of it in a reaction mixture (up to 300 g/L per dry compound). Russian commercial enzyme preparations are used for hydrolysis. The best seems to be Agroxil Plus, which has high cellulase and endoxylanase activities. A total of 290 g/L of sugars (including 210 g/L of glucose and 30 g/L of xylose) is obtained using Agroxil Plus (20 mg protein/1 g substrate) in combination with an auxiliary β-glucosidase enzyme preparation (2 mg protein/1 g substrate) at an initial semi-bleached cellulose concentration of 300 g/L. The dosage of Agroxil Plus can be halved (10 mg of protein/1 g of substrate with a total concentration of semi-bleached cellulose of 300 g/L) with a high yield of hydrolysis product (270 g/L of sugars, including 200 g/L of glucose and 30 g/L of xylose), due to the fractional addition of a substrate.