Induction of micronuclei in mouse bone marrow after combined X-rays-cyclophosphamide and X-rays-mitomycin C treatments

• Published in: Teratogenesis, carcinogenesis, and mutagenesis Vol. 19; no. 4; pp. 267 - 274
• Main Authors: Dobrzyńska, Malgorzata M., Gajewski, Antoni K.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 1999; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - radiation effects; combined treatment; cyclophosphamide; Cyclophosphamide - toxicity; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Drug toxicity and drugs side effects treatment; Erythroid Precursor Cells - cytology; Erythroid Precursor Cells - drug effects; Erythroid Precursor Cells - radiation effects; Male; Medical sciences; Mice; Mice, Inbred Strains; micronuclei; Micronucleus Tests; Miscellaneous (drug allergy, mutagens, teratogens...); Mitomycin - toxicity; mitomycin C; Pharmacology. Drug treatments; Radiation therapy and radiosensitizing agent; Time Factors; Treatment with physical agents; Treatment. General aspects; Tumors; Whole-Body Irradiation; X-Rays; Antineoplastic agent; Mitomycin; Micronucleus test; Mutagenicity testing; Toxicity; Rodentia; Red blood cell; X ray; Radiotherapy; X ray irradiation; Vertebrata; Chemotherapy; Cyclophosphamide; Oxazaphosphinane derivatives; Mammalia; Mouse; Animal; DNA; Nitrogen mustard; Combined treatment; Comparative study
• ISSN: 0270-3211; 1520-6866
• DOI: 10.1002/(SICI)1520-6866(1999)19:4<267::AID-TCM3>3.0.CO;2-#

The induction of micronuclei in polychromatic erythrocytes of bone marrow of Pzh:SWISS mice after combined treatment with X‐rays and cyclophosphamide (CP) or X‐rays and mitomycin C (MMC) were investigated. Combinations of high (1.00 Gy + 100 mg/kg bw CP and 1.00 Gy + 5.25 mg/kg bw MMC) and low (0.25 Gy + 25 mg/kg bw CP and 0.25 Gy + 1.75 mg/kg bw MMC) doses were used. Both chemicals enhanced the mutagenic effects caused by irradiation. After combined treatment with high doses of X‐rays + CP and X‐rays + MMC at different sample times increases in frequency of micronuclei were observed. Mutagenic effects were found also after treatment with two low doses, when irradiation alone produced no effects. The effects of combined treatments are generally similar to the additive effect of the single treatments. Teratogenesis Carcinog. Mutagen. 19:267–274, 1999. © 1999 Wiley‐Liss, Inc.