Alterations in Toll-Like Receptor 7 and -9 mRNA Levels in Lungs after Ovariohysterectomy in a Pyometra Mouse Model

• Published in: European surgical research Vol. 63; no. 2; p. 85
• Main Authors: Kyriakopoulos, Konstantinos, Katsimpoulas, Michael, Mylonas, Konstantinos S, Lidoriki, Irene, Ziogas, Ioannis A, Perivolioti, Efstathia P, Stamataki, Despoina K, Chrelias, Charalampos, Schizas, Dimitrios, Alexandrou, Andreas, Liakakos, Theodoros, Kapelouzou, Alkistis
• Format: Journal Article
• Language: English
• Published: Switzerland 2022
• Subjects: Animals; Disease Models, Animal; Female; Humans; Lung - metabolism; Lung Injury - metabolism; Lung Injury - pathology; Mice; Mice, Inbred C57BL; Myeloid Differentiation Factor 88 - genetics; Myeloid Differentiation Factor 88 - metabolism; NF-kappa B - genetics; NF-kappa B - metabolism; Pyometra - metabolism; Pyometra - pathology; RNA, Messenger; Toll-Like Receptor 7 - metabolism; Toll-Like Receptor 9 - genetics; Toll-Like Receptor 9 - metabolism; Nuclear factor-kappa B; Sepsis; Pyometra; MYD88; Toll-like receptor
• ISSN: 1421-9921
• DOI: 10.1159/000519425

Pyometra (P) leads to sepsis and multiple organ dysfunction syndrome. Toll-like receptors (TLRs) recognize pathogens which can cause P. The aim of this study was to investigate TLR-7 and -9 via the MYD88 pathway and the nuclear factor kappa B (NFκB) response in the uterus of a P mouse model before and after ovariohysterectomy (RP) as well as potential lung injury.
200 female C57BL/6J mice were randomly divided into groups (N = 10/subgroup; sham 1, 2, 3, 7; P1, 2, 3, 7; 1RP1, 2, 3, 7; 2RP1, 2, 3, 7; 3RP1, 2, 3, 7) according to the day of euthanasia. Pathogens were administrated in the groups P and RP in order to induce P.
Alterations in blood chemistry, histopathology, and RT-qPCT analysis before (P) and after RP were observed. Significant correlations were also found between MYD88, NFκB, and TLR9 in P and RP groups in the lungs and in RP groups in the uterus, suggesting that the immune system responded via the TLR9-MYD88 pathway.
This is the first report of immunohistochemical TLR-7 and -9 localization and of TLR-7, -9, MYD88, and NFκB mRNA expression in the uterus causing lung injury in a P mouse model.