Lipid Deficiency Contributes to Impaired Alveolar Progenitor Cell Function in Aging and Idiopathic Pulmonary Fibrosis

• Published in: American journal of respiratory cell and molecular biology Vol. 71; no. 2; p. 242
• Main Authors: Liang, Jiurong, Huang, Guanling, Liu, Xue, Zhang, Xuexi, Rabata, Anas, Liu, Ningshan, Fang, Kai, Taghavifar, Forough, Dai, Kristy, Kulur, Vrishika, Jiang, Dianhua, Noble, Paul W
• Format: Journal Article
• Language: English
• Published: United States 01-08-2024
• Subjects: Aging - metabolism; Aging - pathology; Alveolar Epithelial Cells - metabolism; Alveolar Epithelial Cells - pathology; Animals; Bleomycin; Female; Humans; Idiopathic Pulmonary Fibrosis - metabolism; Idiopathic Pulmonary Fibrosis - pathology; Lipid Metabolism; Male; Mice; Mice, Inbred C57BL; PPAR gamma - metabolism; Stem Cells - metabolism; Stem Cells - pathology; alveolar progenitor cells; aging; idiopathic pulmonary fibrosis; three-dimensional organoid culture; lipid metabolism
• ISSN: 1535-4989
• DOI: 10.1165/rcmb.2023-0290OC

Idiopathic pulmonary fibrosis (IPF) is an aging-associated interstitial lung disease resulting from repeated epithelial injury and inadequate epithelial repair. Alveolar type II cells (AEC2s) are progenitor cells that maintain epithelial homeostasis and repair the lung after injury. In the current study, we assessed lipid metabolism in AEC2s from human lungs of patients with IPF and healthy donors, as well as AEC2s from bleomycin-injured young and old mice. Through single-cell RNA sequencing, we observed that lipid metabolism-related genes were downregulated in IPF AEC2s and bleomycin-injured mouse AEC2s. Aging aggravated this decrease and hindered recovery of lipid metabolism gene expression in AEC2s after bleomycin injury. Pathway analyses revealed downregulation of genes related to lipid biosynthesis and fatty acid β-oxidation in AEC2s from IPF lungs and bleomycin-injured, old mouse lungs compared with the respective controls. We confirmed decreased cellular lipid content in AEC2s from IPF lungs and bleomycin-injured, old mouse lungs using immunofluorescence staining and flow cytometry. Futhermore, we show that lipid metabolism was associated with AEC2 progenitor function. Lipid supplementation and PPARγ (peroxisome proliferator activated receptor γ) activation promoted progenitor renewal capacity of both human and mouse AEC2s in three-dimensional organoid cultures. Lipid supplementation also increased AEC2 proliferation and expression of
in AEC2s. In summary, we identified a lipid metabolism deficiency in AEC2s from lungs of patients with IPF and bleomycin-injured old mice. Restoration of lipid metabolism homeostasis in AEC2s might promote AEC2 progenitor function and offer new opportunities for therapeutic approaches to IPF.