Improvement of adventitious organogenesis for regeneration of transgenic plants in blackberry

• Published in: Genetika (Beograd) Vol. 47; no. 2; pp. 599 - 608
• Main Authors: Gajdosová, Alena, Vujovic, Tatjana, Súkeníková, Miroslava, Libiaková, Gabriela
• Format: Journal Article
• Language: English
• Published: 2015
• Subjects: Agrobacterium tumefaciens; Rubus fruticosus
• ISSN: 0534-0012; 1820-6069
• DOI: 10.2298/GENSR1502599G

The introduction of foreign DNA into the plant genome by Agrobacterium
tumefaciens is a promising technique of targeted gene transfer which depends
on good working regeneration system. The aim of the work was to elaborate the
system for efficient adventitious organogenesis and transgenic plant
regeneration in Rubus fruticosus L. using explants from mature plants.
Regeneration of putative transgenic shoots took place from flag explants
cultivated vertically on MS medium with 1 mg l-1 TDZ and 0.02 mg l-1 IBA
followed by transfer on MS medium with 1 mg l-1 BAP, 0.02 mg l-1 IBA and 0.1
mg l-1 GA3 supplemented with 10-15 mg l-1 hygromycin after transformation by
A. tumefaciens strain LBA 4404 carrying plasmid pCambia 1304. Four putative
transgenic plants of cv. 'Cacanska Bestrna' were rooted and acclimatized.