Tengunoibacter tsumagoiensis gen. nov., sp. nov., Dictyobacter kobayashii sp. nov., Dictyobacter alpinus sp. nov., and description of Dictyobacteraceae fam. nov. within the order Ktedonobacterales isolated from Tengu-no-mugimeshi, a soil-like granular mass of micro-organisms, and emended descriptions of the genera Ktedonobacter and Dictyobacter

• Published in: International journal of systematic and evolutionary microbiology Vol. 69; no. 7; pp. 1910 - 1918
• Main Authors: Wang, Chiung-Mei, Zheng, Yu, Sakai, Yasuteru, Toyoda, Atsushi, Minakuchi, Yohei, Abe, Keietsu, Yokota, Akira, Yabe, Shuhei
• Format: Journal Article
• Language: English
• Published: England 01-07-2019
• Subjects: Dictyobacteraceae; Ktedonobacteria; Tengu-no-mugimeshi
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003396

Three mesophilic, Gram-stain-positive, aerobic bacterial strains, designated Uno3
, Uno11
and Uno16
, were isolated from a soil-like granular micro-organism mass (termed Tengu-no-mugimeshi) collected from Tsumagoi, Gunma, Japan. They grow at 11-37 °C and pH 4.0-8.0, form branched mycelia, and have a G+C content between 49.4-50.3 mol%. The major menaquinone and fatty acid of Uno3
are MK-9 and iso-C16 : 0, respectively, whereas Uno11
and Uno16
share MK-9 (H2) and C16 : 1-2OH. The major cell-wall sugars are mannose (Uno3
and Uno11
) and glucose (Uno16
). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these three strains belong to the order Ktedonobacterales and are most closely related to Dictyobacter aurantiacus S-27
(sequence similarity of 91.3, 96.4 and 95.5 %). Average nucleotide identity values were <79.9 % among Uno11
, Uno16
and D. aurantiacus S-27
, well below the 95-96 % species circumscription threshold. Based on phenotypic features and phylogenetic positions, we propose that Uno3
represents a novel genus and species, Tengunoibacter tsumagoiensis gen. nov., sp. nov. (type strain Uno3
=NBRC 113152
=LMG 30471
=BCRC 81113
) within the new family Dictyobacteraceae fam. nov. Strains Uno11
and Uno16
are also considered to represent novel species: Dictyobacterkobayashii sp. nov. (type strain Uno11
=NBRC 113153
=LMG 30472
=BCRC 81114
) and Dictyobacteralpinus sp. nov. (type strain Uno16
=NBRC 113154
=BCRC 81115
). We also propose an emended description of the genus Dictyobacter, classifying it within family Dictyobacteraceae, and provide emended descriptions of the genera Dictyobacter and Ktedonobacter.