Overview of Characterizing Cancer Glycans with Lectin-Based Analytical Methods

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1928; p. 389
• Main Authors: Pearson, Amanda J, Gallagher, Elyssia S
• Format: Journal Article
• Language: English
• Published: United States 2019
• Subjects: Biological Assay - methods; Biomarkers; Chromatography, Affinity; Glycosylation; Humans; Lectins - chemistry; Lectins - metabolism; Microarray Analysis; Neoplasms - metabolism; Polysaccharides - chemistry; Polysaccharides - metabolism; Lectin; Glycosylation; Microarray; Affinity chromatography; Glycan; Cancer
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-9027-6_20

Glycosylation is a post-translational modification that is often altered in disease development and progression, including cancer. In cancerous patients, the abnormal expression of glycosylation enzymes leads to aberrant glycosylation, which has been linked to malignant tissues. Due to aberrant glycosylation, the presence of specific glycans can be used as biomarkers for identifying the type and stage of cancer. Glycan structures are heterogeneous, with different protein glycoforms having different functional activities. Lectins are an important tool in glycan analysis due to their specificity in binding to unique glycan linkages and monosaccharide units, which allows for the identification of unique glycan structural properties. In this review, we will discuss the use of lectins in microarrays and chromatography for characterizing glycan structures.