Altered Hippocampal Synaptic Potentiation in P2X4 Knock-Out Mice

• Published in: The Journal of neuroscience Vol. 26; no. 35; pp. 9006 - 9009
• Main Authors: Sim, Joan A, Chaumont, Severine, Jo, Jihoon, Ulmann, Lauriane, Young, Mark T, Cho, Kwangwook, Buell, Gary, North, R. Alan, Rassendren, Francois
• Format: Journal Article
• Language: English
• Published: United States Soc Neuroscience 30-08-2006; Society for Neuroscience
• Subjects: Animals; Brief Communications; Hippocampus - physiology; Immunohistochemistry; Ivermectin - pharmacology; Long-Term Potentiation - drug effects; Long-Term Potentiation - physiology; Mice; Mice, Knockout; Receptors, Purinergic P2 - deficiency; Receptors, Purinergic P2 - drug effects; Receptors, Purinergic P2 - physiology; Receptors, Purinergic P2X4; Synapses - physiology; Synaptic Transmission - drug effects
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/JNEUROSCI.2370-06.2006

P2X4 purinergic receptors are calcium-permeable, ATP-activated ion channels. In the CA1 area of the hippocampus, they are located at the subsynaptic membrane somewhat peripherally to AMPA receptors. The possible role of P2X4 receptors has been difficult to elucidate because of the lack of selective antagonists. Here we report the generation of a P2X4 receptor knock-out mouse and show that long-term potentiation (LTP) at Schaffer collateral synapses is reduced relative to that in wild-type mice. Ivermectin, which selectively potentiates currents at P2X4, was found to increase LTP in wild-type mice but had no effect in P2X4 knock-out mice. We suggest that calcium entry through subsynaptic P2X4 receptors during high-frequency stimulation contributes to synaptic strengthening.