Haponin (EIF1AD) interacts with glyceraldehyde 3-phosphate dehydrohenase in the CHO-K1 cell line

• Published in: Russian journal of bioorganic chemistry Vol. 36; no. 3; pp. 287 - 292
• Main Authors: Rakitina, T. V, Bogatova, O. V, Smirnova, E. V, Pozdeev, V. I, Kostanyan, I. A, Lipkin, V. M
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01-05-2010; SP MAIK Nauka/Interperiodica
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; CHO-K1; eIF1AD; GAPDH; haponin; Life Sciences; Organic Chemistry; protein-protein interaction; protein-protein interaction; eIF1AD; GAPDH; CHO-K1; haponin
• ISSN: 1068-1620; 1608-330X
• DOI: 10.1134/S1068162010030027

Haponin (HLDF-alike protein) was previously identified from the human promyelocytic leukemia HL-60 cell line. For the functional study of this protein, we obtained recombinant haponin with an N-terminal hexahistidine tag using a baculovirus expression system. Antibodies against 6xHis-haponin were produced, and the expression of endogenous haponin was demonstrated in mammalian cell lines of different origin. Using affinity chromatography and immunoprecipitation methods, we have shown that in CHO-K1 cells haponin interacts with glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which is one of the vital glycolytic enzymes with a diverse set of noncanonical functions.