Interrelation between structural state, physicochemical properties and lipid composition in microbial cells

• Published in: Biophysics (Oxford) Vol. 58; no. 1; pp. 72 - 77
• Main Authors: Shishkina, L. N., Kapich, A. N., Men’shov, V. A., Goloshchapov, A. N.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 2013; Springer Nature B.V
• Subjects: Anatomy & physiology; Basidiomycetes; Biological and Medical Physics; Biophysics; Cell Biophysics; Cells; Flectobacillus major; Fomes fomentarius; Lipids; Microorganisms; Physics; Physics and Astronomy; Piptoporus betulinus; Pseudomonas fluorescens; Renobacter vacuolatum; lipids; gram-negative bacteria; peroxidation; EPR; xylotrophic basidiomycetes
• ISSN: 0006-3509; 1555-6654
• DOI: 10.1134/S0006350913010144

We performed complex investigations of antioxidant properties and the lipid composition of the five gram-negative bacteria depending on the cultivation season. Structural state, the lipid composition and physicochemical properties of lipids of the three gram-negative bacteria (
Renobacter vacuolatum
,
Flectobacillus major
WKM 869,
Pseudomonas fluorescens
) during their growth and mycelium of four species of the xylotrophic basidiomycetes (
Panus tigrinus
IBK-131,
Fomes fomentarius
M71,
Laetiporus sulfureus
M131,
Piptoporus betulinus
M60) during the lag phase were also studied. Changes in the composition of the lipid component in the studied bacteria led to considerable structural rearrangements in their membrane system during the growth of bacteria. It is shown that more hydrophilic regions of the lipid component are of paramount importance in maintaining the fluidity of the entire lipid bilayer in the basidiomycete mycelium. The scale and direction of the revealed interrelations between rotational correlation times of probes and the lipid composition depend both on localization of the probe in membrane and the composition and physicochemical properties of the microorganism lipids.