Biocompatibility and biological activity of C70 fullerene adduct with L-threonine (C70(C4H9NO3)2)

• Published in: Biochemical and biophysical research communications Vol. 636; pp. 50 - 56
• Main Authors: Sharoyko, Vladimir V., Serebriakov, Evgenii B., Ageev, Sergei V., Petrov, Andrey V., Meshcheriakov, Anatolii A., Charykov, Nikolay A., Murin, Igor V., Maistrenko, Dmitrii N., Molchanov, Oleg E., Semenov, Konstantin N.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 25-12-2022
• Subjects: Biocompatibility; Biological activity; DPPH; Fullerene; HSA; l-threonine; Fullerene; l-threonine; Biocompatibility; DPPH; Biological activity; HSA
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2022.10.054

The aim of this work is to synthesise and study the biocompatibility and biological activity of the C70 fullerene adduct with l-threonine (C70-Thr). The obtained adduct was identified using a complex of physicochemical methods, namely, 13C NMR spectroscopy, IR spectroscopy, thermogravimetric analysis, electron spectroscopy, elemental analysis, and high-performance liquid chromatography. The study of biocompatibility and biological activity of the C70-Thr adduct included the study of haemocompatibility (haemolysis, platelet aggregation, plasma coagulation haemostasis, binding to human serum albumin, esterase activity), antiradical activity, cytotoxicity, cell proliferation, and interaction with DNA (determination of the DNA binding constant and genotoxicity).
•C70 Fullerene adduct with l-threonine (C70-Thr) was synthesised.•C70-Thr binds to human serum albumin and can be applied as a drug carrier.•C70-Thr has low genotoxicity and haemocompatible.•C70-Thr possesses pronounced antioxidant properties in the reaction with DPPH radical.