Fractional recovery of oleaginous bioactive produced by Rhodotorula mucilaginosa CCT3892 using deep eutectic solvents

• Published in: Bioresource technology reports Vol. 12; p. 100561
• Main Authors: da Costa, Willyan Araújo, de Araújo Padilha, Carlos Eduardo, de Medeiros Dantas, Júlia Maria, Silva, Maylla Maria Correia Leite, de Oliveira Rocha, Hugo Alexandre, da Silva, Flávio Luiz Honorato, Ferrari, Márcio, dos Santos, Everaldo Silvino
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-12-2020
• Subjects: Carotenoids; Fractional recovery; Oil-lipids; Yeast cells and deep eutectic solvents; Oil-lipids; Fractional recovery; Carotenoids; Yeast cells and deep eutectic solvents
• ISSN: 2589-014X; 2589-014X
• DOI: 10.1016/j.biteb.2020.100561

The use of four choline chloride-based deep eutectic solvents (DES) in the recovery of oleaginous compounds from Rhodotorula mucilaginosa CCT3892 cells were investigated in this study, focusing on a novelty treatment to recovery mini-compounds intercellular produced by the yeast, as alternative for conventional methods. Regarding DES ability to improve the yeast permeability for an eventual oil-lipids recovery, it was noticed that all solvents were as efficient as a hydrochloride acid treatment, presenting a recovery percentage above 100.0% in relation untreated cells extraction. The best total carotenoid recovery was achieved using DES synthetized with choline chloride an glycerol (ChGy treatment) (0.110 ± 0.005 mg.mL−1). DES promoted a fractional recovery of carotenoids being such process for R. mucilaginosa mentioned for the first time in this paper. Thus, showing potential to be used in the industry once the solvent preserved the antioxidant activity of carotenoids and exhibit moderated cytotoxicity.
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•Deep eutectic solvent (DES) released lipo-compounds from yeast cells.•DES acts in yeast cell disruption or as diffusional recovery agent.•DES are able to act as fractional agents for the intracellular carotenoids recovery.•The obtained extract showed antioxidant activity and low cytotoxicity.