application of DEAE -cellulose column chromatography to the selection of S+ revertants and determination of reversion frequency in populations of S- mutants of fowl plague virus

• Published in: Journal of general virology Vol. 17; no. 1; pp. 49 - 54
• Main Authors: Germanov, A.B, Sokolov, M.I, Parasiuk, N.A
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01-10-1972
• Subjects: animal diseases; animal health; Animals; Chick Embryo; Chromatography, DEAE-Cellulose; Culture Media; Culture Techniques; Evaluation Studies as Topic; Fibroblasts; Genetics, Microbial; Hemagglutination Tests; Hydroxylamines; Influenza A virus - growth & development; Influenza A virus - isolation & purification; Mutagens; Mutation; Protamines; Sodium Chloride
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-17-1-49

Ivanovsky Institute of Virology, Moscow, USSR
A method has been developed for selection of revertants present at low frequency in a population of fowl plague virus. It was based on the differences in elution from DEAE-cellulose columns of the original large-plaque variant (S + ) and polycation-dependent plaque-size mutants (S - ). It was possible to estimate low frequencies of revertants in this way.
Received 24 February 1972;
accepted 8 June 1972.