Assessment of the genotoxic potential of soil-associated DDTs and its metabolites using the Salmonella/microsome assay

• Published in: BIO web of conferences Vol. 100; p. 3016
• Main Authors: Turganova, Ronagul, Koishekenova, Gulshat, Zaypanova, Saule, Delannoy, Matthieu, Jurjanz, Stefan, Bekmanov, Bakhytzhan
• Format: Journal Article
• Language: English
• Published: EDP Sciences 08-04-2024
• Subjects: Agricultural sciences; Agronomy; Animal biology; Animal production studies; Environmental Engineering; Environmental Sciences; Food and Nutrition; Life Sciences; Soil study; Toxicology; Toxicology and food chain; Veterinary medicine and animal Health; Zootechny
• ISSN: 2117-4458; 2273-1709; 2117-4458
• DOI: 10.1051/bioconf/202410003016

The study aimed to assess the genotoxicity of different DDT contaminated soils (with different amendment types) in benzene extracts. A
Salmonella/microsome
assay without metabolic activation (-S9) was performed to evaluate the efficiency to initiate the mutations of remediated and non-remediated soils. The number of revertants mutations in his+ in TA100 and TA98 strains were obtained, and compared to soils without DDT, and remediated DDT containing soils. The interpretation of the data suggests that, mutagenic activity was high for the non-remediated soils samples Kyzylkairat and Beskainar, and revealed in the scale as a weak mutagen. Soil samples treated with DARCO, ORBO, Sarg<150μm, and Sarg>150μm in Kyzylkairat and Beskainar exhibited a decreasing level of mutagenic activity, which is in line with the decreased environmental availability in these soil. Overall, the study indicates that strains TA100 and TA98 are valuable tools for rapidly assessing the potential mutagenicity of soil contaminated with OCPs.