Own Experience From The Use Of A Substitute Of An Allogeneic Acellular Dermal Matrix Revitalized With In Vitro Cultured Skin Cells In Clinical Practice

• Published in: Polski przeglad chirurgiczny Vol. 87; no. 10; pp. 513 - 521
• Main Authors: Łabuś, Wojciech, Kawecki, Marek, Glik, Justyna, Maj, Mariusz, Kitala, Diana, Misiuga, Marcelina, Klama-Baryła, Agnieszka, Kraut, Małgorzata, Nowak, Mariusz
• Format: Journal Article
• Language: English
• Published: Poland De Gruyter Open 01-10-2015
• Subjects: acellular dermal matrix ADM; Acellular Dermis; burn; Burns - surgery; Cicatrix - surgery; Female; fibroblasts; Humans; keratinocytes; Middle Aged; Skin, Artificial; tissue engineering; Tissue Engineering - methods; Transplantation, Autologous; Wound Healing - physiology
• ISSN: 0032-373X; 0032-373X; 2299-2847
• DOI: 10.1515/pjs-2015-0097

As a result of the removal of cells from human allogeneic dermis, a collagen scaffold is obtained, which can be populated de novo with autologous/allogeneic skin cells and transplanted onto the area of skin loss. The optimal method for production of acellular dermal matrices (ADM) has been selected. Three female patients (a mean age of 54 years) were subjected to the transplantation of either autologous or allogeneic keratinocytes and fibroblasts into the holes of acellular dermal matrix (ADM) mesh graft. The method for burn wound treatment based on the use of a viable dermal-epidermal skin substitute (based on ADM and in vitro cultured fibroblasts and keratinocytes) may be the optimal method of burn treatment.