Calcium (Ca2+) expression and intensity in cumulus-oocyte complex (COCs)in Kacang goat after vitrification

• Published in: Polish journal of veterinary sciences Vol. 25; no. 1; pp. 19 - 26
• Main Authors: Widjiati, W, Faizah, Z, Darsini, N, Hendrawan, V F, Karima, H N, Chotimah, C, Sumitro, S B, Yustinasari, L R, Kasman, A A M N, Ntoruru, J M, Luqman, E M
• Format: Journal Article
• Language: English
• Published: Germany Polish Academy of Sciences, Committee of Veterinary Sciences 01-03-2022
• Subjects: Animals; Calcium; Calcium (intracellular); Cold shock; Cryopreservation; Cryopreservation - veterinary; Cumulus Cells - physiology; Cytoplasm; Embryos; Female; Fertilization; Goats; Heat; Heat shock; Immunocytochemistry; In vitro fertilization; Laboratories; Lasers; Oocytes - physiology; Ovaries; Reactive oxygen species; Thawing; Veterinary medicine; Vitrification; Indonesia; confocal laser scanning microscope; food production; Calcium (Ca2+); Kacang goat; cumulus-oocyte complex(COCs)
• ISSN: 1505-1773; 2300-2557
• DOI: 10.24425/pjvs.2022.140836

The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+ in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+ and the intensity of Ca2+ in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+ was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+ in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+ in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+ between fresh COCs and COCs after vitrification. Ca2+ intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.