Whole-genome analysis of human astrocytic tumors by comparative genomic hybridization

• Published in: Brain tumor pathology Vol. 17; no. 1; pp. 21 - 27
• Main Authors: Maruno, M, Ninomiya, H, Ghulam Muhammad, A K, Hirata, M, Kato, A, Yoshimine, T
• Format: Journal Article
• Language: English
• Published: Japan Springer Nature B.V 01-02-2000
• Subjects: Adult; Aged; Astrocytoma - genetics; Brain Neoplasms - genetics; DNA - genetics; Epidermal growth factor; Gene Amplification; Gene Deletion; Gene Dosage; Genome; Glioblastoma - genetics; Humans; Immunohistochemistry; Middle Aged; Nucleic Acid Hybridization; Receptor, Epidermal Growth Factor - metabolism; Tumor Suppressor Protein p53 - metabolism; Tumors
• ISSN: 1433-7398; 1861-387X
• DOI: 10.1007/BF02478914

Frozen sections of 35 astrocytic tumors of various histologic malignancies were analyzed by comparative genomic hybridization in an attempt to characterize the profile of genetic aberrations. Over 94% of the samples revealed DNA copy number aberrations, which increased with higher histological malignancy grades, and also involvement of more than one chromosome was seen in 85% of instances. The aberrations observed were mainly deletions and most frequently incorporated chromosomes 1p, 10, 19q, and 22q. On the other hand, gains or amplifications were detected only in glioblastomas. Additionally, such gains or amplifications were present in all tumor samples where the initial histopathological diagnosis was glioblastoma and immunohistochemical study disclosed p53 tumor suppressor protein negative and epidermal growth factor receptor positive immunoreactivity; such glioblastomas possessing p53 tumor suppressor protein positive and epidermal growth factor receptor negative immunoreactivity seldom displayed any gain. Thus, glioblastomas exhibiting two different profiles of genetic aberrations were recognized--one with and the other without any gains/ amplifications. We speculate that the former variety is de novo glioblastoma.