Understanding the impact of risankizumab on keratinocyte-derived IL-23A in a novel organotypic 3D skin model containing IL-23A responsive and IL-17A producing γδ-T-cells

• Published in: Cutaneous and ocular toxicology Vol. 43; no. 2; pp. 1 - 128
• Main Authors: Huth, Laura, Amann, Philipp M, Marquardt, Yvonne, Jansen, Manuela, Baron, Jens Malte, Huth, Sebastian
• Format: Journal Article
• Language: English
• Published: England 02-04-2024
• Subjects: IL-23/IL-17 axis; γδ-T-cells; IL-36; 3D skin model; anti-IL-23-antibody
• ISSN: 1556-9527; 1556-9535; 1556-9535
• DOI: 10.1080/15569527.2024.2310243

PurposeTo study the effects of the anti-IL-23A antibody risankizumab on the IL-36γ/IL-23A/IL-17A signaling cascade we used a newly developed 3D skin model consisting of primary human keratinocytes, fibroblasts and γδ-T-cells.MethodsIn this
study we developed new full-thickness 3D skin models containing normal human epidermal keratinocytes (NHEK), normal human dermal fibroblasts (NHDF) and IL-23A responsive and IL-17A producing γδ-T-cells. The effects of IL-36γ stimulation with and without risankizumab treatment on IL23A and IL17A expression were examined at the RNA and protein levels.ResultsIn preliminary monolayer experiments stimulation of γδ-T-cells with IL-23A promoted the IL-17A expression that was inhibited after risankizumab treatment. Using 3D skin models containing γδ-T-cells, we found that stimulation with IL-36γ significantly increased not only IL-23A but also IL-17A expression. These effects were inhibited by concomitant treatment with risankizumab.ConclusionsOur results showed that blockade of IL-23A has inhibitory effects on the IL-36γ/IL-23A feedforward loop. Our newly developed 3D skin model containing IL-23A responsive and IL-17A producing γδ-T-cells enables molecular analysis of targeted therapies aimed at the IL-36γ/IL-23A/IL-17A signaling cascade in psoriasis.