Optimization of extraction of bioactive compounds with antiproliferative activity from goji berry and assessment of kinetic models

• Published in: Journal of chemical technology and biotechnology (1986) Vol. 98; no. 12; pp. 2888 - 2903
• Main Authors: Peixoto, Fernanda Barroso, Raimundini Aranha, Ana Caroline, Oliveira Defendi, Rafael, Tonin, Lilian Tatiani Dusman, Sipoli, Caroline Casagrande, Fragoso, Ágatha Giovanna, Düsman, Elisângela, Suzuki, Rúbia Michele
• Format: Journal Article
• Language: English
• Published: Bognor Regis Wiley Subscription Services, Inc 01-12-2023
• Subjects: Anthocyanins; Antioxidants; Antiproliferatives; Bioactive compounds; Biological activity; Carotenoids; Cell viability; Cytotoxicity; Ethanol; Flavonoids; Hepatoma; Lycium barbarum; Optimization; Phenolic compounds; Phenols; Ultrasonic imaging; Ultrasonic methods; Ultrasonic testing; Ultrasound
• ISSN: 0268-2575; 1097-4660
• DOI: 10.1002/jctb.7498

BACKGROUNDThis work aims to optimize the extraction of bioactive compounds with antiproliferative activity from goji berry (Lycium barbarum) and assess the extraction kinetic models. The extraction was performed by varying the proportion of ethanol/water solvent.RESULTSThe antioxidant activity determination tests indicated a significant difference for the proportion of 60:40 ethanol/water. Conditions for the highest levels of antioxidant activity were 45 °C and 6.71 min for ultrasound and 45 °C and 23.30 h for shaker.CONCLUSIONThe quantification of phenolic compounds, flavonoids, carotenoids and anthocyanins showed that the ultrasound method led to the highest concentrations of these compounds. The extraction kinetics was evaluated, and the So and MacDonald's and second‐order models showed the best fit, with a global maximum deviation lower than 10.0%. High concentrations of extracts produced showed cytotoxic effects for human hepatoma cells by the MTT test. The extract produced by ultrasound reached a cell viability of 37.71%. © 2023 Society of Chemical Industry.