Molecular features of mitochondrial DNA in radiation-associated thyroid tumors

• Published in: International Congress series Vol. 1258; pp. 163 - 168
• Main Authors: Rogounovitch, Tatiana I., Saenko, Vladimir A., Shimizu-Yoshida, Yuki, Abrosimov, Aleksandr Yu, Lushnikov, Eugeny F., Roumiantsev, Pavel O., Ohtsuru, Akira, Namba, Hiroyuki, Tsyb, Anatoly F., Yamashita, Shunichi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-11-2003
• Subjects: Common deletion; Large-scale deletions; Mitochondrial DNA; Radiation-associated thyroid tumors; Mitochondrial DNA; Radiation-associated thyroid tumors; Common deletion; Large-scale deletions
• ISSN: 0531-5131; 1873-6157
• DOI: 10.1016/S0531-5131(03)01151-8

DNA samples from tumor and normal thyroid tissues of adult patients with papillary thyroid carcinoma (PTC) and follicular adenoma (FA), who lived in the Chernobyl radioactive zones of Russia and control groups of Japanese patients with PTC, were quantitatively analyzed for the mitochondrial DNA (mtDNA) content and prevalence of the common deletion (CD) and large-scale deletions (LSD). In most tumor tissues, level of mtDNA was elevated. CD and LSD were detected in every sample examined. The quantity of LSD in mtDNA was elevated in most tumor tissues, especially in the radiation-associated group of samples. Also in this group, in the tumor tissue of both PTC and FA, there was a highly significant positive correlation between the number of LSD and the level of relative mtDNA content. In contrast, in sporadic PTCs, this correlation did not occur. Normal tissue samples displayed a statistically insignificant tendency to negative association with relative mtDNA content. Thus, determination of the LSD levels and mtDNA content may be useful for the molecular distinction of radiation-associated and spontaneous thyroid tumors.