IN VITRO PROPERTIES OF NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS FROM A BULGE REGION OF WHISKER FOLLICLE

• Published in: Biotechnologia acta Vol. 7; no. 4; pp. 71 - 79
• Main Authors: Vasyliev, R G, Rodnichenko, A E, Zubov, D A, Labunets, I F, Novikova, S N, Butenko, G M
• Format: Journal Article
• Language: English
• Published: Kyiv Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 01-01-2014; National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
• Subjects: clonogenicity; hair follicle; multipotent stem cells; neural crest; sphere-formation capacity
• ISSN: 2410-7751; 1995-5537; 2410-776X
• DOI: 10.15407/biotech7.04.071

A culture method for multipotent neural crest-derived stem cell isolated from the bulge region of the hair follicle of whisker pad of an adult mice has been described and their biological properties have been studied. It was shown that the cells possess a fibroblast-like morphology, they are nestin-positive and cytokeratin-negative, and also express the following surface markers: CD44, CD73, CD90 and Sca-1. This cell type shows the functional properties of stem cells in culture: clonogenicity, self-renewal, sphereforming capacity and the ability to the directed multilineage differentiation. Due to these properties, neural crest-derived multipotent stem cells are promising for application in the regenerative medicine.