Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA

Human-induced pluripotent stem cells (iPSCs) are showing great promise for both disease modeling and regenerative medicine. The choice of reprogramming methods have a significant effect on the outcomes of the experiments. Standard methods, such as Sendai viruses, episomes, and the base-modified mRNA...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2239; p. 163
Main Author:	Annand, Robert R
Format:	Journal Article
Language:	English
Published:	United States 2021
Subjects:	Cell Culture Techniques - methods Cells, Cultured Cellular Reprogramming - genetics Cryopreservation Culture Media - chemistry Fibroblasts - cytology Fibroblasts - metabolism Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism RNA, Messenger Transfection - methods Cellular reprogramming Human fibroblasts Induced pluripotent stem cells (iPSC) Synthetic mRNA Stem cells
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Summary:	Human-induced pluripotent stem cells (iPSCs) are showing great promise for both disease modeling and regenerative medicine. The choice of reprogramming methods have a significant effect on the outcomes of the experiments. Standard methods, such as Sendai viruses, episomes, and the base-modified mRNA have limitations. Here, I describe a method to reprogram human fibroblasts using a cocktail of mRNAs without any base modification that increases reprogramming efficiency, reduces the RNA-associated toxicity, and yields iPSCs ready for expansion and characterization in as short as 10-14 days.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-1084-8_11