Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions

Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions

Chromatin immunoprecipitation (ChIP) is a method used to examine the genomic localization of a target of interest (e.g., proteins, protein posttranslational modifications, or DNA elements). As ChIP provides a snapshot of in vivo DNA-protein interactions, it lends insight to the mechanisms of gene ex...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 2261; p. 323
Main Authors: Small, Eliza C, Maryanski, Danielle N, Rodriguez, Keli L, Harvey, Kevin J, Keogh, Michael-C, Johnstone, Andrea L
Format: Journal Article
Language:English
Published: United States 2021
Subjects:
Animals
Cells, Cultured
Chromatin - genetics
Chromatin - metabolism
Chromatin Immunoprecipitation
DNA - genetics
DNA - metabolism
Histones - metabolism
Humans
Protein Binding
Protein Processing, Post-Translational
Workflow
Chromatin immunoprecipitation
ChIP normalization
Histone PTMs
Semisynthetic nucleosomes
Antibody specificity
Spike-in controls
ChIP
Recombinant nucleosomes
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Description
Summary:Chromatin immunoprecipitation (ChIP) is a method used to examine the genomic localization of a target of interest (e.g., proteins, protein posttranslational modifications, or DNA elements). As ChIP provides a snapshot of in vivo DNA-protein interactions, it lends insight to the mechanisms of gene expression and genome regulation. This chapter provides a detailed protocol focused on native-ChIP (N-ChIP), a robust approach to profile stable DNA-protein interactions. We also describe best practices for ChIP , including defined controls to ensure specific and efficient target enrichment and methods for data normalization.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1186-9_20