NMR-based comparative metabolomics of quiescent muscle cells

• Published in: Journal of biosciences Vol. 49; no. 2; p. 59
• Main Authors: Purohit, Gunjan, Ramesh, Akila, Patel, Anant B, Dhawan, Jyotsna
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 25-05-2024; Springer Nature B.V
• Subjects: Animals; Biomarkers; Biomedical and Life Sciences; Biomedicine; Catabolism; Cell Biology; Cell culture; Cell Differentiation; Cell Proliferation; Choline; Enrichment; Life Sciences; Magnetic Resonance Spectroscopy - methods; Metabolism; Metabolites; Metabolome; Metabolomics; Metabolomics - methods; Mice; Microbiology; Muscle, Skeletal - cytology; Muscle, Skeletal - metabolism; Muscles; Myoblasts; Myoblasts - cytology; Myoblasts - metabolism; NMR; NMR spectroscopy; Nuclear magnetic resonance; Phosphocholine; Phospholipids; Plant Sciences; Regeneration (biological); Repair; Spectroscopy; Stem cells; Zoology; nuclear magnetic resonance spectroscopy; quiescence; Biomarker; myoblast; G; metabolic profile
• ISSN: 0973-7138; 0250-5991; 0973-7138
• DOI: 10.1007/s12038-024-00442-x

Adult muscle tissue largely comprised of differentiated myofibers also harbors quiescent muscle-resident stem cells (MuSCs) that are responsible for its maintenance, repair and regeneration. Emerging evidence suggests that quiescent MuSCs exhibit a specific metabolic state, which is regulated during physiological and pathological alterations. However, a detailed understanding of the metabolic state of quiescent MuSCs and its alteration during activation and repair is lacking. Direct profiling of MuSCs
in vivo
is challenging because the cells are rare and dispersed, while isolation and enrichment leads to their activation and loss of quiescence. In this study, we employed
1
H-nuclear magnetic resonance
(
NMR) spectroscopy to profile metabolites in an established culture model of quiescent MuSC-derived myoblasts and compared with activated, proliferative and differentiated muscle cells to determine the state-specific metabolome. We report that the proliferating and differentiated cells are highly enriched in metabolites involved in energy generation, the quiescent state is enriched in metabolites related to phospholipid catabolism (glycerophosphocholine and choline) and depleted for phosphocholine which is enriched in proliferating cells. We propose that the ratio of these metabolites may be useful as a biomarker of MuSC quiescence.