Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection

• Published in: European journal of clinical microbiology & infectious diseases Vol. 43; no. 11; pp. 2211 - 2214
• Main Authors: Yahaya, H., Cheah, Y. K., Chee, H. Y., Than, L. T. L.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-11-2024; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Candidiasis; Cross-reactivity; Design; Enzymes; Gene amplification; Health sciences; Infectious diseases; Internal Medicine; Medical laboratories; Medical Microbiology; Microbiology; Morbidity; Mortality; Nucleic acids; Original Article; Yeasts; Invasive candidiasis; LAMP; PCR; Culture; C. glabrata
• ISSN: 0934-9723; 1435-4373; 1435-4373
• DOI: 10.1007/s10096-024-04934-4

Purpose
Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of
C. glabrata
.
Methodology
The specific LAMP primers for
C. glabrata
detection were designed and evaluated.
Results
The LAMP assay accurately detected
C. glabrata
with no cross-reactivity with other
Candida
species.
Conclusion
The developed molecular method would be a promising tool in the management of invasive candidiasis.