Protein Kinase A Inhibitor Attenuates the Antinociceptive Effect of NMDA-Receptor Channel Antagonists in the Capsaicin Test in Mice

• Published in: Bulletin of experimental biology and medicine Vol. 177; no. 2; pp. 231 - 234
• Main Authors: Ivanova, E. A., Vasilchuk, A. G., Voronina, T. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-06-2024; Springer Nature B.V
• Subjects: Analgesics - pharmacology; Animals; Biomedical and Life Sciences; Biomedicine; Capsaicin; Capsaicin - pharmacology; Capsaicin receptors; Cell Biology; Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors; Cyclic AMP-Dependent Protein Kinases - metabolism; Dizocilpine; Dizocilpine Maleate - pharmacology; Drug dosages; Glutamic acid receptors (ionotropic); Internal Medicine; Kinases; Laboratory animals; Laboratory Medicine; Male; Mice; N-Methyl-D-aspartic acid receptors; Nociceptive Pain - chemically induced; Nociceptive Pain - drug therapy; Pain; Pain Measurement - drug effects; Pain Measurement - methods; Pain perception; Pathology; Pharmacology; Phosphorylation; Protein kinase A; Protein kinase A inhibitors; Protein Kinase Inhibitors - pharmacology; Proteins; Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors; Software; Topical application; Toxicology; TRPV Cation Channels - antagonists & inhibitors; TRPV Cation Channels - metabolism; nociceptive pain; NMDA receptor; protein kinase A; mice; TRPV1 ion channel
• ISSN: 0007-4888; 1573-8221; 1573-8221
• DOI: 10.1007/s10517-024-06162-4

Acute nociceptive pain in mice caused by subcutaneous (intraplantar) injection of TRPV1 ion channel agonist capsaicin (1.6 μg/mouse) and the effects of protein kinase A inhibitor H-89 (0.05 mg/mouse, intraplantar injection) and NMDA receptor channel antagonists MK-801 (7.5 and 15 μg/mouse, topical application) and hemantane (0.5 mg/mouse, topical application) on the pain were assessed. MK-801 and hemantane were found to reduce the duration of the pain response. H-89 did not significantly affect the pain in animals, but preliminary administration of this drug abolished the antinociceptive effect of MK-801 (7.5 μg/mouse) and weakens the effect of hemantane (0.5 mg/mouse).