Anti-inflammatory Effect of Symplocos prunifolia Extract in an In Vitro Model of Acute Pneumonia

• Published in: Plant foods for human nutrition (Dordrecht) Vol. 79; no. 4; pp. 893 - 900
• Main Authors: Kim, Seok Woo, Jee, Wona, Park, So-Mi, Park, Ye-Rin, Bae, Hanbit, Na, Yun-Cheol, Lee, Han-Gyul, Kwon, Seungwon, Jang, Hyeung-Jin
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-12-2024; Springer Nature B.V
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Cyclooxygenase-2; Diarrhea; Dysentery; Dyspepsia; Ecology; Food Science; Herbal medicine; Kinases; Lipopolysaccharides; Lung diseases; MAP kinase; Morbidity; NF-κB protein; Nitric oxide; Nitric-oxide synthase; Nuclear transport; Nutrition; Older people; Phosphorylation; Plant Physiology; Pneumonia; Protein expression; Proteins; Respiratory diseases; Skin diseases; Symplocos; Translocation; Ulcers; Waterborne diseases; Natural product; Anti-inflammatory effect; extract; Acute pneumonia; Symplocos prunifolia extract
• ISSN: 0921-9668; 1573-9104; 1573-9104
• DOI: 10.1007/s11130-024-01231-5

Acute pneumonia is a respiratory disease characterized by inflammation within the lung tissue, exhibiting higher morbidity rates and mortality rates among immunocompromised children and older adults.
Symplocos
species have been traditionally used as herbal remedies for conditions like dysentery, skin ulcers, diarrhea, and dyspepsia. Contemporary research has employed various
Symplocos
species in the study of diverse diseases. However, the exact efficacy and mechanisms of action of
Symplocos Prunifolia
remain unknown. Therefore, this study investigated the anti-inflammatory mechanism of
S. prunifolia
extract (SPE) in A549 and RAW264.7 cells stimulated by lipopolysaccharide (LPS). SPE significantly reduced nitric oxide (NO) production and the protein expression levels of like inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 cells. Furthermore, it reduced the protein expression levels of iNOS, COX-2 and the levels of pro-inflammatory cytokines in LPS-stimulated A549 cells. The mechanism underlying the anti-inflammatory effect of SPE was associated with the inhibition of LPS stimulated the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) and Mitogen-activated protein kinase (MAPK) phosphorylation. Moreover, we confirmed that SPE decreased the nuclear translocation of nuclear factor-κB (NF-κB)/p65 stimulated by LPS. In conclusion, these results demonstrate that SPE alleviates inflammatory responses by deactivating the PI3K/Akt, MAPK, and NF-κB signaling pathways. Our findings suggest that SPE is a potential candidate for acute pneumonia prevention.