Relatedness Among Indiginous Members of Sclerotinia sclerotiorum by Mycelial Compatibility and RAPD Analysis in the Jordan Valley

• Published in: The plant pathology journal Vol. 21; no. 2; pp. 106 - 110
• Main Authors: Osofee, H. (Jordan University of Science and Technology, Irbid, Jordan), Hameed, K.M. (Jordan University of Science and Technology, Irbid, Jordan), E-mail: [e-mail], Mahasneh, A. (Jordan University of Science and Technology, Irbid, Jordan)
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 2005
• Subjects: cottony rot; Cucurbita pipo; Jordan valley; mycelial compatibility; RAPD assay; SCLEROTINIA SCLEROTIORUM; cottony rot; RAPD assay; Sclerotinia sclerotiorum; Jordan valley; mycelial compatibility; Cucurbita pipo
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.2005.21.2.106

Sclerotinia sclerotiorum attacks most of the vegetable crops in the Jordan valley. Twenty-five samples/isolates were obtained in a complete coverage of that region. They were characterized for their mycelium incompatibility, and specific gene amplified using the primer SSREV/SSFWD. All isolates gave similar single band around 278 bp. Thirteen isolates were completely incompatible with the other 12 ones. The latter ones fell into four subgroups of mycelium incompatibility. RAPD analysis using three primers (OPA-2, OPA-10, and OPA-18) clustered the 25 isolates into subgroups in agreement with their morphological separation, indicating close correlation between amplified gene(s) and the gene(s) of incompatibility.