Isolation of a human lactoferrin biosimilar from frozen milk of goat producers

• Published in: E3S Web of Conferences Vol. 390; p. 2044
• Main Authors: Mosolova, Natalya I., Petrushko, Elena V., Bogdanovich, Dmitry M., Karpenko, Ekaterina V., Vorontsova, Elena S.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Les Ulis EDP Sciences 01-01-2023
• Subjects: Cleaning process; Degreasing; Filtrate; Goat's milk; Lactoferrin; Mass spectrometry; Mass spectroscopy; Microbiological analysis; Milk; Raw materials
• ISSN: 2267-1242; 2555-0403; 2267-1242
• DOI: 10.1051/e3sconf/202339002044

This study offers an additional source of information on isolation and purification of the human lactoferrin biosimilar from frozen milk of goat producers. Thanks to the application of an improved technique of raw material degreasing, a milk filtrate with complete absence of the fat part was obtained, which as a result had a favorable effect on the yield and properties of the finished product, whose quality was confirmed by methods of MALDI-TOF mass spectrometry and microbiological analysis. As a result 135 g of human lactoferrin biosimilar was obtained from 75 liters of frozen milk.