Light and Fungal Elicitor Induce 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase mRNA in Suspension Cultured Cells of Parsley (Petroselinum crispum L.)

• Published in: Plant physiology (Bethesda) Vol. 98; no. 2; pp. 761 - 763
• Main Authors: Henstrand, John M., McCue, Kent F., Kent Brink, Handa, Avtar K., Herrmann, Klaus M., Conn, Eric E.
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01-02-1992
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biosynthesis; Cell culture techniques; Communications; Cultured cells; Enzymes; Fundamental and applied biological sciences. Psychology; Gene expression regulation; Genes; Kinetics; Messenger RNA; Metabolism; Nitrogen metabolism; Parsley; Plant physiology and development; Shikimate pathway; Phenylalanine ammonia-lyase; Plant pathogen; Transcription; Enzyme; Petroselinum crispum; Phycomycetes; Biosynthesis; Elicitor; Fungi; Suspension culture; Messenger RNA; Dicotyledones; Aminoacid; Light; Angiospermae; Flavoring crop; Spermatophyta; Umbelliferae; Kinetics; Phytophthora megasperma; Biological accumulation; Thallophyta
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.98.2.761

Light and fungal elicitor induce mRNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase in suspension cultured cells of parsley (Petroselinum crispum L.). The kinetics and dose response of mRNA accumulation were similar for DAHP synthase and phenylalanine ammonia-lyase (PAL). Six micrograms of elicitor from Phytophthora megasperma f. glycinia gave a detectable induction within 1 hour. Induction of DAHP synthase and PAL mRNAs by light was transient, reaching maximal levels at 4 hours and returning to pretreatment levels after 24 hours. Our data suggest that either light or fungal elicitor transcriptionally activate DAHP synthase. A coordinate regulation for key enzymes in the synthesis of primary and secondary metabolites is indicated.