Hantavirus: Preliminary assessment of lateral flow immune assay prototypes to detect IgM and IgG antibodies

• Published in: Diagnostic microbiology and infectious disease Vol. 110; no. 4; p. 116521
• Main Authors: Guimarães, João Pedro Tôrres, Fogaça, Matheus Bernardes Torres, Moura, Rodrigo Scaliante, Mendonça, Marcelo, Conceição, Fabricio Rochedo, Gimaque, João Bosco Lima, de Almeida, Talita Heleny Pacheco, Abrain, Cláudia, Mourão, Maria Paula Gomes, Figueiredo, Luiz Tadeu Moraes, Stefani, Mariane Martins Araújo, Bührer-Sékula, Samira
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-12-2024
• Subjects: Diagnostics; Hantavirus; Lateral flow assay; Rapid tests; Serology; Rapid tests; Lateral flow assay; Serology; Hantavirus; Diagnostics
• ISSN: 0732-8893; 1879-0070; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2024.116521

•Preliminary evaluation of three LFIA prototypes for hantavirus antibody detection.•IgM/IgG prototype showed 100 % sensitivity and 99.3 % specificity.•Expanded control group tested for cross-reactivity with similar symptomatic diseases.•LFIA prototypes offer rapid, accessible diagnostics for low-resource settings.•Future optimizations include separate IgM/IgG lines to enhance specificity.
Three lateral flow immunoassay prototypes developed to detect IgM, IgG and IgM/IgG antibodies against Hantavirus were evaluated. A total of 163 samples were tested: 10 from Hantavirus patients, 103 from related diseases, and 50 from healthy controls. The prototypes exhibited 100 % sensitivity, 97.5 % to 99.3 % specificity, indicating promising improved diagnosis.