Phytic acid improves osteogenesis and inhibits the senescence of human bone marrow mesenchymal stem cells under high-glucose conditions via the ERK pathway

• Published in: Chemico-biological interactions Vol. 387; p. 110818
• Main Authors: Liu, Dong-Yu, Wu, Jin, Zhou, He-Yang, Lv, Jia-Xin, Cai, Kun-Zhan, Tang, Chun-Bo
• Format: Journal Article
• Language: English
• Published: Ireland 05-01-2024
• Subjects: Animals; Bone Marrow Cells; Cell Differentiation; Cells, Cultured; Glucose - metabolism; Humans; MAP Kinase Signaling System; Mesenchymal Stem Cells; Osteogenesis; Phytic Acid - metabolism; Phytic Acid - pharmacology; ERK pathway; Cell senescence; Bone marrow mesenchymal stem cells; Osteogenic differentiation; Phytic acid
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2023.110818

Hyperglycaemia causes impairment of osteogenic differentiation and accelerates stem cell senescence, resulting in weakened osteogenesis and disordered bone metabolism. Phytic acid (PA) is an antioxidant that is reportedly beneficial to bone homeostasis. The present study aims to clarify how PA affects the osteogenic capacity and cellular senescence of bone marrow mesenchymal stem cells (BMSCs) exposed to high-glucose environments, as well as the potential molecular mechanisms. Our results indicate that osteogenic differentiation in BMSCs cultivated in high-glucose conditions is enhanced by PA, as evidenced by increased alkaline phosphatase activity and staining, Alizarin Red S staining, osteogenic marker in in vitro studies, and increased osteogenesis in animal experiments. PA also prevented high-glucose-induced senescence of BMSCs, as evidenced by the repression of reactive oxygen species production, senescence-associated β-galactosidase staining, and P21 and P53 expression. Furthermore, it was found that PA rescued the high-glucose-inhibited expression of phosphorylated extracellular regulated protein kinases (p-ERK). The inhibition of ERK pathway by the specific inhibitor PD98059 blocked the PA-enhanced osteogenesis of BMSCs and promoted cell senescence. Our results revealed that PA enhances osteogenic differentiation and inhibits BMSC senescence in a high-glucose environment. In addition, the activation of the ERK pathway seems to mediate the beneficial effects of PA. The findings provide novel insights that could facilitate bone regeneration in patients with diabetes.