UDP glucose: glucan synthetase in developing cotton fibers. iI. structure of the reaction product

UDP glucose: glucan synthetase in developing cotton fibers. iI. structure of the reaction product

The solubility properties, composition, and structure of the radioactive product synthesized from UDP-[14C]glucose by a highly active cotton fiber glucan synthetase have been determined. Product obtained under the following three different conditions was analyzed: at high and low substrate concentra...

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Bibliographic Details
Published in:Plant physiology (Bethesda) Vol. 59; no. 4; pp. 719 - 723
Main Authors: Heiniger, U, Delmer, D.P
Format: Journal Article
Language:English
Published: United States American Society of Plant Physiologists 01-04-1977
Subjects:
Acid hydrolysis
Cell walls
Cotton fibers
Enzymes
Glucans
Methylation
Oxidation
Plants
Polysaccharides
Radioactive decay
uridine diphosphate
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Summary:The solubility properties, composition, and structure of the radioactive product synthesized from UDP-[14C]glucose by a highly active cotton fiber glucan synthetase have been determined. Product obtained under the following three different conditions was analyzed: at high and low substrate concentrations by detached fibers, and at high substrate concentrations with an isolated particulate preparation. The results of acetic and nitric acid digestion, enzyme digestion, total acid hydrolyses, periodate oxidation, partial acid hydrolyses, and methylation analyses all support the conclusion that the product of the glucan synthetase produced under all three assay conditions is a linear β-(1→3)-glucan.
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Present address: Institut de Biologie Végétale et de Phytochimie, Université de Fribourg, 3 Rue Albert-Gockel, CH-1700 Fribourg, Switzerland.
To whom reprint requests should be sent.
Supported by the U.S. Energy Research and Development Administration under Contract E(11-1)-1338.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.59.4.719