Multiplication of HVT FC-126 (Herpesvirus Turkey) virus in the kidney cell lines of NO avian origin

• Published in: Acta veterinaria (Beograd) Vol. 60; no. 4; pp. 381 - 389
• Main Authors: Filipič, M., University of Ljubljana, Ljubljana (Slovenia). Medical Faculty, Gottstein, Ž., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine, Sladoljev, S., Institute of Immunology, Zagreb (Croatia), Cencič, A., University of Maribor, Hoče (Slovenia). Faculty of Agriculture, Koren, S., University of Ljubljana, Ljubljana (Slovenia). Medical Faculty, Ciglar-Grozdanić, I., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine, Mazija, H., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine
• Format: Journal Article
• Language: English
• Published: 2010
• Subjects: CELLS; CELLULE; CELULAS; CERDO; DINDON; ENFERMEDAD DE MAREK; GERBILLE; GERBILS; GERBO; HERPESVIRUS DEL PAVO; HERPESVIRUS DU DINDON; http://www.fao.org/aos/agrovoc#c_1418; http://www.fao.org/aos/agrovoc#c_15596; http://www.fao.org/aos/agrovoc#c_36998; http://www.fao.org/aos/agrovoc#c_4099; http://www.fao.org/aos/agrovoc#c_4599; http://www.fao.org/aos/agrovoc#c_7555; http://www.fao.org/aos/agrovoc#c_8016; KIDNEYS; MALADIE DE MAREK; MAREK'S DISEASE; PAVO; PORCIN; REIN; RINONES; SWINE; TURKEY HERPESVIRUS; TURKEYS
• ISSN: 0567-8315; 1820-7448
• DOI: 10.2298/AVB1004381F

The presented experiments were aimed to cultivate and multiplicate HVT FC-126 in the PLA (Adult pig kidney) and GL-4 (Gerbil kidney) cell lines. Two different HVT FC-126 vaccines strains were used: Marikal SPF (Veterina d.o.o., Croatia) and Lyomarex (Merial, USA). They were adapted to the PLA and GL-4 cell lines. After adaptation, they were titrated on PLA and GL-4. On both cell lines, they show similar CPE (cytopathic effect). The difference between them was detected using Real Time PCR, which was also positive by agarose gel analysis for the virus contained in Lyomarex, but not in the Marikal SPF. It can be concluded that both cell lines are sensitive to HVT FC-126 and the virus can be multiplied in high titers though much lower than in the calf infestation epithelial cell line (CIEB) cells.