Coding strategy of the S and M genomic segments of a hantavirus representing a new subtype of the Puumala serotype

• Published in: Archives of virology Vol. 140; no. 11; pp. 2011 - 2026
• Main Authors: Reip, A, Haring, B, Sibold, C, Stohwasser, R, Bautz, E.K.F, Darai, G, Meisel, H, Kruger, D.H
• Format: Journal Article
• Language: English
• Published: Wien Springer 1995; New York, NY
• Subjects: Amino Acid Sequence; amino acid sequences; Antigens, Viral - genetics; Base Sequence; Biological and medical sciences; classification; DNA, Complementary - genetics; epitopes; Fundamental and applied biological sciences. Psychology; genbank/u14136; genbank/u14137; Genes, Viral; genetic variation; Genetics; Hantavirus; Hantavirus - classification; Hantavirus - genetics; Microbiology; Molecular Sequence Data; nucleotide sequences; Phylogeny; Proteins - genetics; RNA, Viral - genetics; Sequence Alignment; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; strain differences; Viral Core Proteins - genetics; Viral Structural Proteins - genetics; Virology; zoonoses; Virus; Bunyaviridae; Complementary DNA; Phylogenetic tree; Sequence alignment; Homology; Hantavirus; Genome; Comparative study; Aminoacid sequence; Structural analysis; Strain
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/BF01322689

The hantavirus strain Vranica was previously reported to have been isolated from a bank vole in Bosnia-Hercegovina and associated with the occurrence of hemorrhagic fever with renal syndrome (HRFS) in humans. The complete cDNA nucleotide sequences of the small (S) and medium (M) genomic RNA segments of this virus were determined. Major open reading frames were found in the S and M segment between nucleotide positions 43 and 1341 coding for a polypeptide of 433 amino acid residues and between nucleotide positions 41 and 3 484 coding for 1148 amino acid residues, respectively. The analysis and the alignment of the nucleotide and the derived amino acid sequences with known sequences of other hantavirus strains demonstrate that Vranica resembles Swedish strains and represents a new virus subtype of the Puumala serotype distinct from the subtypes represented by virus strains CG18-20 and Sotkamo.