The invasive margin of early-stage human colon tumors is infiltrated with neutrophils of an antitumoral phenotype

• Published in: Journal of leukocyte biology Vol. 114; no. 6; pp. 672 - 683
• Main Authors: Vadillo, Eduardo, Mantilla, Alejandra, Aguilar-Flores, Cristina, De León-Rodríguez, Saraí Gisel, Vela-Patiño, Sandra, Badillo, Juan, Taniguchi-Ponciano, Keiko, Marrero-Rodríguez, Daniel, Ramírez, Lourdes, León-Vega, Iliana Itzel, Fuentes-Castañeda, Carmen, Piña-Sánchez, Patricia, Prieto-Chávez, Jessica Lakshmi, Pérez-Kondelkova, Vadim, Montesinos, Juan José, Bonifaz, Laura, Pelayo, Rosana, Mayani, Héctor, Schnoor, Michael
• Format: Journal Article
• Language: English
• Published: England 24-11-2023
• Subjects: Colonic Neoplasms - pathology; Humans; Intercellular Adhesion Molecule-1 - metabolism; Neutrophils - metabolism; Phenotype; CD95; ICAM-1; polarization; cancer progression; CXCR2
• ISSN: 1938-3673; 1938-3673
• DOI: 10.1093/jleuko/qiad123

Neutrophils infiltrate several types of cancer; however, whether their presence is associated with disease progression remains controversial. Here, we show that colon tumors overexpress neutrophil chemoattractants compared to healthy tissues, leading to their recruitment to the invasive margin and the central part of colon tumors. Of note, tumor-associated neutrophils expressing tumor necrosis factor α, which usually represents an antitumoral phenotype, were predominantly located in the invasive margin. Tumor-associated neutrophils from the invasive margin displayed an antitumoral phenotype with higher ICAM-1 and CD95 expression than neutrophils from healthy adjacent tissues. A higher neutrophil/lymphocyte ratio was found at later stages compared to the early phases of colon cancer. A neutrophil/lymphocyte ratio ≤3.5 predicted tumor samples had significantly more neutrophils at the invasive margin and the central part. Moreover, tumor-associated neutrophils at the invasive margin of early-stage tumors showed higher ICAM-1 and CD95 expression. Coculture of colon cancer cell lines with primary neutrophils induced ICAM-1 and CD95 expression, confirming our in situ findings. Thus, our data demonstrate that tumor-associated neutrophils with an antitumoral phenotype characterized by high ICAM-1 and CD95 expression infiltrate the invasive margin of early-stage colon tumors, suggesting that these cells can combat the disease at its early courses. The presence of tumor-associated neutrophils with antitumoral phenotype could help predict outcomes of patients with colon cancer.