Kurloff cell proteoglycans: Evidence for de novo synthesis of chondroitin sulphate proteoglycans by purified Kurloff cells

• Published in: FEBS letters Vol. 209; no. 2; pp. 299 - 304
• Main Authors: Landemore, Gérard, Letaïef, Slim-Eric, Bocquet, Jean, Izard, Jacques
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-12-1986; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Chondroitin sulfate; Chondroitin Sulfate Proteoglycans - biosynthesis; Chondroitin Sulfate Proteoglycans - isolation & purification; Fundamental and applied biological sciences. Psychology; Glucosamine - metabolism; Glycoproteins; Glycosaminoglycan; Guinea Pigs; Kurloff cell, Spleen cell; Leukocyte; Molecular Weight; Proteins; Proteoglycan; Proteoglycans - biosynthesis; Spleen - cytology; Spleen - metabolism; Sulfates - metabolism; Sulfur Radioisotopes; Tritium; Proteoglycan; Glycosaminoglycan; Chondroitin sulfate; Kurloff cell, Spleen cell; Leukocyte; Biosynthesis
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)81131-0

This paper reports the first direct demonstration of de novo synthesis ofchondroitin sulphate proteoglycans by Kurloff cells. This was achieved using highly purified splenic Kurloff cells labelled in vitro with [
35S]sulphate and D-[U-
3H]glucosamine. A single population of sulphated proteoglycans was observed after dissociative extraction, DEAE-cellulose chromatography, Sepharose CL 6B chromatography and fluorography after electrophoresis. These were large, highly anionic proteoglycans and were completely digested by chondroitinase AC or ABC. Moreover, glycosaminoglycan extracted from Kurloff cells had the electrophoretic mobility of control chondroitin sulphate.