Anti-inflammatory potential of lactic acid bacteria from southern Brazil by reducing TNF-α and increasing IL-10 synthesis: an in vitro study

• Published in: Journal of applied microbiology Vol. 134; no. 8
• Main Authors: Vincenzi, Angélica, Goettert, Márcia Inês, Volken de Souza, Claucia Fernanda
• Format: Journal Article
• Language: English
• Published: England 01-08-2023
• Subjects: Animals; Anti-Inflammatory Agents; Brazil; Interleukin-10; Lactobacillales; Milk - microbiology; Probiotics; Tumor Necrosis Factor-alpha; Brazil; Lacticaseibacillus paracasei; Probiotics; p38/MAPK; cytokines; Lactiplantibacillus plantarum
• ISSN: 1365-2672; 1365-2672
• DOI: 10.1093/jambio/lxad168

To evaluate the adhesion capacity and anti-inflammatory activity of lactic acid bacteria (LAB) isolated from raw cow milk and artisan cheese in Southern Brazil, investigating their effect on the release of cytokines such as TNF-α and IL-10 and their influence on the activation of the p38/MAPK pathway.
Lentilactobacillus parabuchneri ML4, Lacticaseibacillus paracasei ML33, Lactiplantibacillus pentosus ML82, Lactiplantibacillus plantarum CH131, and L. paracasei CH135 demonstrated high adhesion potential in an in vitro model of the intestinal epithelium, as well as anti-inflammatory activity. After a 4-hour treatment, the strains significantly increased TNF-α levels, while a 24-hour treatment led to a significant decrease in TNF-α release. Moreover, IL-10 levels significantly increased after 24-hour and 48-hour treatments with LAB. The inhibition of p38/MAPK phosphorylation was identified as one of the mechanisms by which the L. paracasei ML33 and L. plantarum CH131 strains suppressed the production and release of TNF-α.
We identified LAB strains with potential anti-inflammatory properties that could adhere to the intestinal mucosa and alleviate the inflammatory response by reducing the production and release of TNF-α through the inhibition of the p38/MAPK pathway, while promoting the production of IL-10.