An analysis of the bovine genome by density-gradient centrifugation

• Published in: European journal of biochemistry Vol. 84; no. 1; pp. 179 - 188
• Main Authors: Macaya, G, Cortades, J, Bernadi, G. (Institut de Recherche en Biologie Moleculaire, Paris (France). Lab. de Genetique Moleculaire)
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-03-1978
• ISSN: 0014-2956; 1432-1033
• DOI: 10.1111/j.1432-1033.1978.tb12155.x

The dG+dC‐rich fractions obtained by density gradient centrifugation of bovine DNA in Cs2SO4/BAMD [J. Cortadas, G. Macaya & G. Bernardi (1977) Eur. J. Biochem. 76, 13–19] were centrifuged in Cs2SO4/Ag+ density gradients. These experiments led to the preparation of the DNA components which had been detected (by analytical centrifugation in CsCl) in the Cs2SO4/BAMD fractions, and also of DNA components which had identical behaviors in Cs2SO4/BAMD gradients and identical buoyant densities in CsCl. A total of eight satellite components and 11 minor components, accounting for 23% and 4% of the bovine genome, respectively, were thus isolated and characterized in their relative amounts and buoyant densities. The implications of these results on the interpretation of renaturation kinetic data on the bovine genome are discussed.