Association between PSCA, TNF-α, PARP1 and TP53 Gene Polymorphisms and Gastric Cancer Susceptibility in the Brazilian Population

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 21; no. 1; pp. 43 - 48
• Main Authors: Dantas, Roberto Nery, Souza, Augusto Monteiro de, Herrero, Sylvia Satomi Takeno, Kassab, Paulo, Malheiros, Carlos Alberto, Lima, Eleonidas Moura
• Format: Journal Article
• Language: English
• Published: Thailand West Asia Organization for Cancer Prevention 01-01-2020
• Subjects: Adenocarcinoma - genetics; Alleles; Antigens, Neoplasm - genetics; Biomarkers, Tumor - genetics; Brazil; Case-Control Studies; Female; Gene Frequency - genetics; Genetic Predisposition to Disease - genetics; Genotype; GPI-Linked Proteins - genetics; Humans; Male; Middle Aged; Neoplasm Proteins - genetics; Poly (ADP-Ribose) Polymerase-1 - genetics; Polymorphism, Single Nucleotide - genetics; Retrospective Studies; Stomach Neoplasms - genetics; Tumor Necrosis Factor-alpha - genetics; Tumor Suppressor Protein p53 - genetics; Brazil; Molecular markers; Single nucleotide polymorphism; Genotyping; Gastric cancer
• ISSN: 1513-7368; 2476-762X
• DOI: 10.31557/APJCP.2020.21.1.43

To evaluate the association of allelic and genotypic frequencies of PSCA (rs2976392), TNF-α (rs1800629), PARP1 (rs1136410) and TP53 (rs368771578) SNPs with GC susceptibility in a Brazilian population.
This is a retrospective study, which included 102 paraffin-embedded adenocarcinoma tissue samples > 5 years of obtention, with 204 alleles for each studied SNP. Other 102 healthy tissue samples were included as controls. For analysis, the genotyping method Dideoxy Single Allele-Specific - PCR was used. Statistical analysis was performed with the Bioestat software 5.3, determining Hardy-Weinberg's equilibrium for the genotypic frequencies p-values < 0.05 were considered significant.
PSCA (rs2976392) and TNF-α (rs1800629) SNPs were associated with GC in the analyzed samples (X2=10.3/102 and p<0.001/0.00001, respectively). TNF-α (rs1800629) SNP presented also a statistically significant relationship between its genotypes and the morphological pattern (intestinal/diffuse) (p<0.032). However, PARP1 (rs1136410) and TP53 (rs368771578) SNPs were in Hardy-Weinberg's equilibrium and, therefore, were not significantly associated with GC in these samples (X2=0.73/2.89 and p<0.39/0.08).
PSCA (rs2976392) and TNF-α (rs1800629) SNPs are potential molecular markers of susceptibility to GC development. PARP1 (rs1136410) and TP53 (rs368771578) SNPs were not associated with the risk of GC development.