Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stress

• Published in: Plant biotechnology reports Vol. 16; no. 6; pp. 697 - 707
• Main Authors: Chen, Siting, Qiu, Guanglong
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01-12-2022; Springer Nature B.V
• Subjects: 14-3-3 protein; Abiotic stress; Agriculture; Arabidopsis; Biomedical and Life Sciences; Biotechnology; Cell Biology; Enzymatic activity; Life Sciences; Original Article; Osmosis; Osmotic pressure; Osmotic stress; Plant Biochemistry; Plant Sciences; Reactive oxygen species; Salinity tolerance; Salts; Structure-function relationships; Water loss; Seagrass; Salt stress; -; Osmotic stress
• ISSN: 1863-5466; 1863-5474
• DOI: 10.1007/s11816-022-00795-5

Salinity affects the physiological activities of seagrass by changing the osmotic pressure of seagrass cells, which then affects the structure and function of seagrass community. In this study, we created transgenic Arabidopsis that overexpressed the
ZjGRF1
gene from the intertidal seagrass
Zostera japonica
. We found that transgenic
ZjGRF1
Arabidopsis has stronger tolerance to salt stress and osmotic stress than wild type. Under salt stress, the content of reactive oxygen species in transgenic
ZjGRF1
Arabidopsis was lower than that in wild-type Arabidopsis. The viability of root cells in transgenic
ZjGRF1
Arabidopsis was higher than that of wild type under salt stress. Under salt stress, transgenic
ZjGRF1
Arabidopsis accumulated more proline and had higher antioxidant enzyme activities. The rate of water loss rate from overexpressed plants was lower than that of the wild-type plants. The resistance of overexpressed plants to osmotic stress may be enhanced by the ABA-dependent pathway. Our results confirm that
ZjGRF1
enhances tolerance to salt and osmotic stress in
Z
.
japonica
.