Assessment of potential pathogenicity among clinical strains of Pseudomonas aeruginosa using bioluminescent technique

• Published in: Bi͡u︡lletenʹ Sibirskoĭ medit͡s︡iny Vol. 11; no. 4; pp. 44 - 48
• Main Authors: M. V. Kuznetsova, I. L. Maslennikova, T. I. Karpunina, N. V. Nikolayeva
• Format: Journal Article
• Language: English
• Published: Siberian State Medical University (Tomsk) 28-08-2012
• Subjects: e. coli lux; p. aeruginosa; биолюминесценция; патогенность
• ISSN: 1682-0363; 1819-3684
• DOI: 10.20538/1682-0363-2012-4-44-48

The aim: to study the influence of P. aeruginosa exometabolites on E. coli lux+ bioluminescence and experimentally prove the expediency of use of a bioluminescent method for an estimation of their potential pathogenicity. Objectives and Methods. Exometabolites of P. aeruginosa (n = 27) nosocomial strains were used. Index of potential pathogenicity (IPP) was calculated with the formula: IPP = (Ik — Io)/Ik × 100%, where Ik — intensity of Escherichia coli lux+ bioluminescence in control test (LB), Io — intensity of a bioluminescence in experimental test (exometabolites). Results. Protocol of analysis implementation and exposure duration has been determined. According to IPP, P. aeruginosa strains were subdivided into 3 groups: strains with low degree of pathogenicity, moderate and highly pathogenic. The group of IPP ³ 70% was characterized by reliably high correlation coefficient relatively to pyocyanin production, film-forming ability. This indicates the possibility of bioluminescent method application as screening or additional method of the assessment of P. aeruginosa isolate aggressiveness, and in purulent-septic infections (PSI) in particular.