Effect of Essential Oil of Piper tuberculatum var. minus C.DC. Leaves on the Hepatic Microsomal 7-Ethoxyresorufin O-Deethylase (EROD) and 7-Ethoxycoumarin O-Deethylase (ECOD) Activity in Rats

• Published in: Journal of essential oil-bearing plants (Dehra Dun) Vol. 18; no. 2; pp. 329 - 334
• Main Authors: Dias dos Santos, Paulo Roberto, Gomes Pereira, Flaviane, Marques Freitas, José Carlos, Melo dos Santos, Nataly, de Albuquerque Farinelle, Cristiane, Bosco de Salles, João, von Sohsten de Souza Medeiros, Erika, Franklin Guimarães, Elsie, Coelho Kaplan, Maria Auxiliadora
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 04-03-2015
• Subjects: Enzymatic activity; enzyme activity; essential oils; germacrene; germacrene B; germacrene D; hydrodistillation; leaves; oils; Piper; Piperaceae; rats; sesquiterpenes
• ISSN: 0976-5026; 0972-060X; 0976-5026
• DOI: 10.1080/0972060X.2014.885229

The essential oil of Brazilian Piper tuberculatum var. minus (Piperaceae) was analyzed and its effect on two activities of cytochrome P- EROD (CYP1A) and ECOD (CYP2A) were evaluated employing hepatic microsomal EROD and ECOD of rats. The oil was extracted by hydrodistillation technique on modified Clevenger-type apparatus. The major compound of the essential oil was germacrene D, germacrene B and iso - caryophyllene were also identified. The essential oil inhibited about 60 % of EROD activity in vitro with 12.5 µL per mL of reaction medium but failed to inhibit the ECOD activity even with 100 µL per mL of reaction medium. The study on enzymatic activities with the essential oil of this species showed that there was EROD inhibition, but no ECOD inhibition.