Effects of Nd3+ on Calcium-Dependent Processes in Isolated Rat Heart Mitochondria and Frog Heart Muscle

• Published in: Biochemistry (Moscow). Supplement series A, Membrane and cell biology Vol. 13; no. 2; pp. 161 - 167
• Main Authors: Korotkov, S. M., Sobol, C. V., Schemarova, I. V., Furaev, V. V., Novozhilov, A. V., Nesterov, V. P.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-04-2019; Springer Nature B.V
• Subjects: 2,4-Dinitrophenol; Amphibians; Biomedical and Life Sciences; Calcium; Calcium ions; Calcium permeability; Cardiac muscle; Cell Biology; Contraction; Electron transport; Heart; Life Sciences; Membrane permeability; Mitochondria; Mitochondrial permeability transition pore; Muscles; Myocardium; Neodymium; Rare earth elements; Respiration; Rodents; Swelling; Vertebrates; inotropic effect; contractility; Nd; Ψ; myocardium; Ca; mitochondrial respiration
• ISSN: 1990-7478; 1990-7494
• DOI: 10.1134/S1990747819070018

The inotropic and chronotropic effects of neodymium ions (Nd
3+
) on the heart muscle of a frog
Rana ridibunda
and the influence of Nd
3+
on respiration, swelling, and potential (∆Ψ
mito
) of Ca
2+
-loaded rat heart mitochondria (RHM) were studied. It was found that Nd
3+
reduced the amplitude and frequency of spontaneous heart contraction (
F
max
); Nd
3+
also prevented a short-term increase in Ca
2+
-induced basal respiration of mitochondria and their swelling in salt media, as well as a decrease of ∆Ψ
mito
on the inner mitochondrial membrane (IMM). At the same time, Nd
3+
slightly affected mitochondrial respiration in state 3 or in 2,4-dinitrophenol (DNP)-uncoupled state. These effects of Nd
3+
may indicate that Nd
3+
inhibits the mitochondrial permeability transition pore (MPTP) opening, which is formed in calcium loaded mitochondria. The data obtained are important for a better understanding of the mechanisms of action of rare earth elements on Ca
2+
-dependent processes in the myocardium of vertebrates.